Regulatory

Part:BBa_K4012002:Design

Designed by: TIANLANG CHEN   Group: iGEM21_AISSU_Union   (2021-09-28)


pGAL1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 547
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 163
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3
    Illegal BsaI.rc site found at 557


Design Notes

CrCPR is a enhancement mode for the following expression of the sequences. So we choose a induced promoter.


Source

Saccharomyces pastorianus strain CBS 1483 chromosome ScII

References